Human Alpha1 Antichymotrypsin

Mfr.No.

E1073Hu

Sensitivity

5.17ug/ml

Detection Range

10-3500ug/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Alpha1 Antichymotrypsin (also known as AACT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ACT;Alpha-1-antichymotrypsin;Alpha-1-antichymotrypsin His-Pro-less;Cell growth-inhibiting gene 24/25 protein;Serpin A3;SERPINA 3;SERPINA3

Molecular Weight

47651

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1073Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	538.6	29.75	5.5
Intra-Assay	2	18	1460.6	72.58	5
Intra-Assay	3	18	395.7	20.72	5.2

Background

Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

Protocol

These standard curves of E1073Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ug/ml	2.134	2.121	2.064
	2.108
1000ug/ml	1.431	1.442	1.386
	1.454
500ug/ml	0.676	0.779	0.723
	0.882
250ug/ml	0.402	0.476	0.419
	0.55
125ug/ml	0.32	0.298	0.241
	0.275
0ug/ml	0.074	0.057	0
	0.04

Gene ID	12
UniProt ID	P01011

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102726	E1073Hu	48T	$320		Add to Cart
BHE12102726	E1073Hu	96T	$458		Add to Cart

Human Alpha1 Antichymotrypsin - AACT ELISA Kit

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