Human Alpha-methylacyl-CoA Recemase

Mfr.No.

E3387Hu

Description

This sandwich kit is for the accurate quantitative detection of human Alpha-methylacyl-CoA Recemase (also known as AMACR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

2-methylacyl-CoA racemase;Alpha-methylacyl-CoA racemase;AMACR

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

42387

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.16ng/L

Background

Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed: 7649182, PubMed: 10655068, PubMed: 11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed: 7649182, PubMed: 10655068, PubMed: 11060359).

Gene ID	23600
UniProt ID	Q9UHK6
Antigen	AMACR

Research Area

Others

Protocol

These standard curves of E3387Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.436	2.436	2.389
	2.436
1200ng/L	1.433	1.412	1.364
	1.39
600ng/L	0.944	0.928	0.881
	0.912
300ng/L	0.487	0.464	0.417
	0.442
150ng/L	0.255	0.251	0.204
	0.248
0ng/L	0.042	0.047	0
	0.052

Precision

We measured random samples of E3387Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	545.4	30.62	5.6
Intra-Assay	2	18	1506.3	52.96	3.5
Intra-Assay	3	18	496.3	31.3	6.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104915	E3387Hu	48T	$320		Add to Cart
BHE12104915	E3387Hu	96T	$458		Add to Cart

Human Alpha-methylacyl-CoA Recemase - AMACR ELISA Kit

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