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Home / ELISA Kit

Human Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3) ELISA Kit

SKU : BHE13703174

AFP-L3 is one of AFP fractions which is synthesized by malignant cell and comprise special oligosaccharide.It is a new generation of tumor marker for HCC and yields useful information on HCC for clinical decision making. Reports at home or abroad have showed that if the ratio of AFP-L3 increased over 10%, the risk of catching HCC had increased highly, and non-malignant diseases hardly synthesize this AFP fraction(AFP-L3).AFP-L3 can be detected 9-12 months earlier and shows the remarkable precaution effect to HCC. AFP-L3 is the fucosylated variant of AFP produced specifically by HCC. AFP-L3 bears strong affinity to the sugar chain of Lens culinaris, a type of lentil. AFP-L3 is less sensitive but more specific than AFP, and therefore cannot replace AFP. The value of AFP-L3 lies mainly in distinguishing HCC from other conditions when AFP is elevated.

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Mfr.No.	KTE60958
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3) ELISA Kit employs a two-site sandwich ELISA to quantitate AFP-L3.
Alternative Names	AFP-L3
Detection Method	Colorimetric
Kit Components	Human Alpha-fetoprotein Lens culinaris agglutiin 3 microplate Human Alpha-fetoprotein Lens culinaris agglutiin 3 standard Human Alpha-fetoprotein Lens culinaris agglutiin 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3) ELISA Kit has high sensitivity and excellent specificity for detection of Human AFP-L3. No significant cross-reactivity or interference between Human AFP-L3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	AFP-L3 is one of AFP fractions which is synthesized by malignant cell and comprise special oligosaccharide.It is a new generation of tumor marker for HCC and yields useful information on HCC for clinical decision making. Reports at home or abroad have showed that if the ratio of AFP-L3 increased over 10%, the risk of catching HCC had increased highly, and non-malignant diseases hardly synthesize this AFP fraction(AFP-L3).AFP-L3 can be detected 9-12 months earlier and shows the remarkable precaution effect to HCC. AFP-L3 is the fucosylated variant of AFP produced specifically by HCC. AFP-L3 bears strong affinity to the sugar chain of Lens culinaris, a type of lentil. AFP-L3 is less sensitive but more specific than AFP, and therefore cannot replace AFP. The value of AFP-L3 lies mainly in distinguishing HCC from other conditions when AFP is elevated.
Notes	This Human Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3) ELISA Kit employs a two-site sandwich ELISA to quantitate AFP-L3 in samples. An antibody specific for AFP-L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAFP-L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFP-L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFP-L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3) ELISA Kit employs a two-site sandwich ELISA to quantitate AFP-L3 in samples. An antibody specific for AFP-L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAFP-L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFP-L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFP-L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703174	KTE60958	48 T	$359		Add to Cart
BHE13703174	KTE60958	96 T	$619		Add to Cart
BHE13703174	KTE60958	96 Tx5	$2809		Add to Cart
BHE13703174	KTE60958	96 Tx50	$26209		Add to Cart

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