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Home / ELISA Kit

Human Alpha-Actinin 1 - ACTN1 ELISA Kit

SKU : BHE12107786

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

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Bioassay Technology Laboratory

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Sample Request

Mfr.No.

E6424Hu

Sensitivity

0.032ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ACTN-1;Alpha-actinin cytoskeletal isoform;Alpha-actinin-1;BDPLT15;F-actin cross-linking protein;Non-muscle alpha-actinin-1

Molecular Weight

103058

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E6424Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.21	0.09	7.64
Intra-Assay	2	18	4.61	0.22	4.79
Intra-Assay	3	18	1.8	0.12	6.47

Background

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Protocol

These standard curves of E6424Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.269	2.303	2.259
	2.336
6ng/mL	1.608	1.655	1.611
	1.701
3ng/mL	1.186	1.089	1.045
	0.992
1.5ng/mL	0.777	0.729	0.685
	0.68
0.75ng/mL	0.422	0.414	0.371
	0.406
0ng/mL	0.057	0.044	0
	0.03

Gene ID	87
UniProt ID	P12814

Research Area	Signal Transduction; Stem Cells; Developmental Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107786	E6424Hu	48T	$320		Add to Cart
BHE12107786	E6424Hu	96T	$458		Add to Cart

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