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Home / ELISA Kit

Human Alpha-2-macroglobulin-like protein 1 (A2ML1) ELISA Kit

SKU : BHE13703058

The alpha-macroglobulin (AM) superfamily of proteins contains both complement components and protease inhibitors, including A2M and A2ML1. AM proteins display a unique trap mechanism of inhibition, by which the AM inhibitor undergoes a major conformational change upon its cleavage by a protease, thus trapping the protease and blocking it from subsequent substrate binding. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates. Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

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Mfr.No.	KTE60842
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Alpha-2-macroglobulin-like protein 1 (A2ML1) ELISA Kit employs a two-site sandwich ELISA to quantitate A2ML1.
Alternative Names	A2ML1; CPAMD9; DKFZp686C1729; DKFZp686D2011; DKFZp686G1812; DKFZp686L1821; DKFZp686O1010; FLJ16045; FLJ25179; FLJ39129; FLJ41597; FLJ41598; FLJ41607; C3 and PZP-like; alpha-2-macroglobulin domain containin
Detection Method	Colorimetric
Kit Components	Human Alpha-2-macroglobulin-like protein 1 microplate Human Alpha-2-macroglobulin-like protein 1 standard Human Alpha-2-macroglobulin-like protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Alpha-2-macroglobulin-like protein 1 (A2ML1) ELISA Kit has high sensitivity and excellent specificity for detection of Human A2ML1. No significant cross-reactivity or interference between Human A2ML1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The alpha-macroglobulin (AM) superfamily of proteins contains both complement components and protease inhibitors, including A2M and A2ML1. AM proteins display a unique trap mechanism of inhibition, by which the AM inhibitor undergoes a major conformational change upon its cleavage by a protease, thus trapping the protease and blocking it from subsequent substrate binding. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates. Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Notes	This Human Alpha-2-macroglobulin-like protein 1 (A2ML1) ELISA Kit employs a two-site sandwich ELISA to quantitate A2ML1 in samples. An antibody specific for A2ML1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyA2ML1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A2ML1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A2ML1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	144568

Applications	ELISA
Applications Notes	This Human Alpha-2-macroglobulin-like protein 1 (A2ML1) ELISA Kit employs a two-site sandwich ELISA to quantitate A2ML1 in samples. An antibody specific for A2ML1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyA2ML1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A2ML1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A2ML1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703058	KTE60842	48 T	$339		Add to Cart
BHE13703058	KTE60842	96 T	$589		Add to Cart
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BHE13703058	KTE60842	96 Tx50	$24569		Add to Cart

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