Human AGP1/alpha 1 acid glycoprotein ELISA Kit PicoKine®

Mfr.No.

EK1486

Sensitivity

<150 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.001	0.064	0.164	0.409	0.747	1.387	1.633	1.750

Description

Human AGP1/alpha 1 acid glycoprotein ELISA Kit PicoKine® (96 Tests). Quantitate Human ORM1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Alpha-1-acid glycoprotein 1;AGP 1;Orosomucoid-1;OMD 1;ORM1;AGP1;

Molecular Weight

23512 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-S201

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human AGP1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2696	12095	38792	2753	12889	38542
Standard deviation	164.45	931.31	1784.43	192.71	1198.67	2235.43
CV (%)	6.1%	7.7%	4.6%	7%	9.3%	5.8%

Protein Function

Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. .

Background

Alpha-1-acid glycoprotein, or orosomucoid, is a relatively abundant plasma protein synthesized in the liver. It has a molecular weith of 40,000 Da and is a single polypeptide chain of about 180 amino acids. AGP is considered an acute phase reactant; its plasma levels increase several fold during acute infections. It belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. And this gene is located in 9q34.3-qter.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2696	2424	2599	2810	2632	141.53	5.3%
Sample 2	12095	11209	12283	11042	11657	539.13	4.6%
Sample 3	38792	45455	38596	41095	40984	2761.77	6.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5004
Gene Name	ORM1
UniProt ID	P02763
Protein Name	Alpha-1-acid glycoprotein 1
Tissue Specificity	Expressed by the liver and secreted in plasma.

Applications	ELISA
Research Area	Cardiovascular,Immunology,Innate Immunity,Macrophage/Inflammation,Secreted Molecules,Serum Proteins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001120	EK1486	96 wells/kit	$499		Add to Cart

Human AGP1/alpha 1 acid glycoprotein ELISA Kit PicoKine®

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