Human Aggrecan/ACAN ELISA Kit PicoKine®

Mfr.No.

EK0909

Sensitivity

<50 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.001	0.023	0.052	0.109	0.235	0.512	1.033	1.887

Description

Human Aggrecan ELISA Kit PicoKine® (96 Tests). Quantitate Human ACAN in cell culture supeatants, serum and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Aggrecan core protein;Cartilage-specific proteoglycan core protein;CSPCP;Chondroitin sulfate proteoglycan core protein 1;Chondroitin sulfate proteoglycan 1;Aggrecan core protein 2;ACAN;AGC1, CSPG1, MSK16;

Molecular Weight

250193 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: V20-R675

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Aggrecan

Subcellular Localization

Secreted, extracellular space, extracellular matrix .

Sample Type

Cell culture supeatants, Serum, Urine

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	3084	13064	43102	3322	13982	40208
Standard deviation	240.55	679.32	1939.59	275.72	908.83	1809.36
CV (%)	7.8%	5.2%	4.5%	8.3%	6.5%	4.5%

Protein Function

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Background

Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	3084	3011	3191	2954	3060	88.56	2.8%
Sample 2	13064	11683	11338	13036	12280	779.41	6.3%
Sample 3	43102	43347	44289	42847	43396	544.9	1.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	176
Gene Name	ACAN
UniProt ID	P16112
Protein Name	Aggrecan core protein
Tissue Specificity	Restricted to cartilages. .

Applications	ELISA
Research Area	Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Mesenchymal Stem Cells,Signal Transduction,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000981	EK0909	96 wells/kit	$450		Add to Cart

Human Aggrecan/ACAN ELISA Kit PicoKine®

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