Human Aftiphilin - AFTPH ELISA Kit

Mfr.No.

E2821Hu

Sensitivity

10.21ng/L

Detection Range

20-6000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Aftiphilin;AFTPH

Molecular Weight

102113

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2821Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2116.4	58.43	2.8
Intra-Assay	2	18	1618.4	71.02	4.4
Intra-Assay	3	18	1172.6	38.17	3.3

Background

Component of clathrin-coated vesicles (PubMed: 15758025). Component of the aftiphilin/p200/gamma-synergin complex, which plays roles in AP1G1/AP-1-mediated protein trafficking including the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (PubMed: 15758025).

Protocol

These standard curves of E2821Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	1.688	1.794	1.753
	1.9
1600ng/L	0.928	0.967	0.926
	1.006
800ng/L	0.495	0.528	0.487
	0.561
400ng/L	0.164	0.154	0.113
	0.144
200ng/L	0.086	0.076	0.035
	0.066
0ng/L	0.034	0.041	0
	0.048

Gene ID	54812
UniProt ID	Q6ULP2

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104358	E2821Hu	48T	$320		Add to Cart
BHE12104358	E2821Hu	96T	$458		Add to Cart

Human Aftiphilin - AFTPH ELISA Kit

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