Human Afamin ELISA Kit PicoKine®

Mfr.No.

EK1487

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.030	0.149	0.251	0.480	0.751	1.261	1.806	2.089

Description

Human Afamin ELISA Kit PicoKine® (96 Tests). Quantitate Human AFM in cell culture supeatants, serum and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Afamin;Alpha-albumin;Alpha-Alb;AFM;ALB2, ALBA;

Molecular Weight

69069 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L22-N599

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Afamin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Urine

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	221	658	3779	249	672	4128
Standard deviation	10.83	33.56	219.18	17.68	51.07	321.98
CV (%)	4.9%	5.1%	5.8%	7.1%	7.6%	7.8%

Protein Function

Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. .

Background

Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	221	236	257	237	237	12.79	5.3%
Sample 2	658	655	603	647	640	22.16	3.4%
Sample 3	3779	3418	3836	3710	3685	160.89	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	173
Gene Name	AFM
UniProt ID	P43652
Protein Name	Afamin
Tissue Specificity	High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level). .

Applications	ELISA
Research Area	Cardiovascular,Cofactors, Vitamins/Minerals,Metabolism,Pathways and Processes,Serum Proteins,Signal Transduction,Vitamins/Minerals

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001263	EK1487	96 wells/kit	$750		Add to Cart

Human Afamin ELISA Kit PicoKine®

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