Human Adp Ribosylation Factor Gtpase Activating Protein 3

Mfr.No.

E7279Hu

Sensitivity

2.19ng/L

Detection Range

5-1800ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ADP-ribosylation factor GTPase-activating protein 3;ARF GAP 3;ARFGAP1;ARFGAP3

Molecular Weight

56928

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E7279Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	572.6	11.44	2
Intra-Assay	2	18	217.36	12.32	5.67
Intra-Assay	3	18	299.78	12.86	4.29

Background

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.

Protocol

These standard curves of E7279Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1000ng/L	2.367	2.311	2.268
	2.255
500ng/L	1.218	1.218	1.176
	1.218
250ng/L	0.706	0.746	0.703
	0.785
125ng/L	0.378	0.392	0.35
	0.406
62.5ng/L	0.201	0.163	0.121
	0.126
0ng/L	0.052	0.043	0
	0.033

Gene ID	26286
UniProt ID	Q9NP61

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115252	E7279Hu	48T	$320		Add to Cart
BHE12115252	E7279Hu	96T	$458		Add to Cart

Human Adp Ribosylation Factor Gtpase Activating Protein 3 - ARFGAP3 ELISA Kit

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