Human Adiponectin ELISA Kit PicoKine®

Mfr.No.

EK0595

Sensitivity

<60 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.014	0.105	0.170	0.326	0.596	0.979	1.507	1.967

Description

Human Adiponectin ELISA Kit PicoKine® (96 Tests). Quantitate Human ADIPOQ in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 60pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Adiponectin ;Adiponectin, C1Q and collagen domain containing ;cDNA FLJ78108, highly similar to Homo sapiens adiponectin, C1Q and collagen domain containing (ADIPOQ), mRNA ;ADIPOQ ;hCG_1784052 ;

Molecular Weight

26414 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-N244

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human total Adiponectin

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	3726	12058	49927	3646	11478	49590
Standard deviation	182.57	940.52	2346.56	236.99	1033.02	2578.68
CV (%)	4.9%	7.8%	4.7%	6.5%	9%	5.2%

Background

Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	3726	3810	3849	3865	3812	53.79	1.4%
Sample 2	12058	12135	11622	10357	11543	712.13	6.1%
Sample 3	49927	48196	52754	50855	50433	1645.09	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	9370
Gene Name	ADIPOQ
UniProt ID	A8K660
Protein Name	Adiponectin

Applications	ELISA
Research Area	Heart Disease,Lipids/Lipoproteins,Mesenchymal Stem Cells,Metabolism,Metabolism Processes,Neurology Process,Neuroscience,Obesity,Pathways and Processes,Response To Hypoxia,Signal Transduction,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000540	EK0595	96 wells/kit	$499		Add to Cart

Human Adiponectin ELISA Kit PicoKine®

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