Human Adenosylhomocysteinase

Mfr.No.

E4910Hu

Sensitivity

9.97ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Cytidine Deaminase (also known as CDA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Adenosylhomocysteinase;AdoHcyase;AHCY;S-adenosyl-L-homocysteine hydrolase

Molecular Weight

47716

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4910Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1498.6	65.99	4.4
Intra-Assay	2	18	219.3	16.69	7.6
Intra-Assay	3	18	415.1	31.23	7.5

Background

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.

Protocol

These standard curves of E4910Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.379	2.365	2.305
	2.351
1200ng/L	1.566	1.584	1.525
	1.602
600ng/L	1.164	1.129	1.069
	1.094
300ng/L	0.648	0.713	0.653
	0.778
150ng/L	0.417	0.44	0.38
	0.462
0ng/L	0.057	0.06	0
	0.062

Gene ID	191
UniProt ID	P23526

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106183	E4910Hu	48T	$320		Add to Cart
BHE12106183	E4910Hu	96T	$458		Add to Cart

Human Adenosylhomocysteinase - AHCY ELISA Kit

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