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Home / ELISA Kit

Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit

SKU : BHE13703141

ADAMTSL1 encodes a secreted protein resembling members of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) family. This protein lacks the propeptide region and the metalloproteinase and disintegrin-like domains, which are typical of the ADAMTS family, but contains other ADAMTS domains, including the thrombospondin type 1 motif. This protein may have important functions in the extracellular matrix. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.The deduced 525-amino acid protein contains an N-terminal signal peptide. The mature secreted form contains 497 amino acids and has a calculated molecular mass of about 55 kD.

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Mfr.No.	KTE60925
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTSL1.
Alternative Names	ADAMTSL1; RP11-220B22.2; ADAMTSR1; C9orf94; DKFZp686L03130; FLJ35283; FLJ41032; FLJ46891; MGC118803; MGC118805; MGC40193; PUNCTIN; ADAM-TS related protein 1
Detection Method	Colorimetric
Kit Components	Human ADAMTS-like protein 1 microplate Human ADAMTS-like protein 1 standard Human ADAMTS-like protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAMTSL1. No significant cross-reactivity or interference between Human ADAMTSL1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ADAMTSL1 encodes a secreted protein resembling members of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) family. This protein lacks the propeptide region and the metalloproteinase and disintegrin-like domains, which are typical of the ADAMTS family, but contains other ADAMTS domains, including the thrombospondin type 1 motif. This protein may have important functions in the extracellular matrix. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.The deduced 525-amino acid protein contains an N-terminal signal peptide. The mature secreted form contains 497 amino acids and has a calculated molecular mass of about 55 kD.
Notes	This Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTSL1 in samples. An antibody specific for ADAMTSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTSL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	92949

Applications	ELISA
Applications Notes	This Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTSL1 in samples. An antibody specific for ADAMTSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTSL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703141	KTE60925	48 T	$339		Add to Cart
BHE13703141	KTE60925	96 T	$589		Add to Cart
BHE13703141	KTE60925	96 Tx5	$2629		Add to Cart
BHE13703141	KTE60925	96 Tx50	$24569		Add to Cart

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