Human ADAM8/Ms2 ELISA Kit PicoKine®

Mfr.No.

EK0650

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.006	0.06	0.129	0.256	0.442	0.829	1.384	1.979

Description

Human ADAM8/Ms2 ELISA Kit PicoKine® (96 Tests). Quantitate Human ADAM8 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Disintegrin and metalloproteinase domain-containing protein 8;ADAM 8;3.4.24.-;Cell surface antigen MS2;CD156a;ADAM8;MS2;

Molecular Weight

88771 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-P497

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ADAM8

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	143	493	1805	150	511	1879
Standard deviation	10.72	97.47	97.47	11.25	33.72	127.77
CV (%)	7.5%	5.6%	5.4%	7.5%	6.6%	6.8%

Protein Function

Possible involvement in extravasation of leukocytes.

Background

A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	143	150	153	145	147	3.96	2.6%
Sample 2	493	533	465	527	504	27.43	5.4%
Sample 3	1805	1971	1907	1936	1904	61.88	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	101
Gene Name	ADAM8
UniProt ID	P78325
Protein Name	Disintegrin and metalloproteinase domain-containing protein 8
Tissue Specificity	Expressed on neutrophils and monocytes.

Applications	ELISA
Research Area	Apoptosis,Cancer,Cell Biology,Cell Death,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Metalloprotease,Proteolysis/Ubiquitin,Proteolytic Enzymes,Receptors,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001006	EK0650	96 wells/kit	$499		Add to Cart

Human ADAM8/Ms2 ELISA Kit PicoKine®

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