Human ADAM12 / MCMP ELISA Kit PicoKine®

Mfr.No.

EK0934

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.027	0.084	0.138	0.264	0.491	0.831	1.340	2.070

Description

Human ADAM12 / MCMP ELISA Kit PicoKine® (96 Tests). Quantitate Human ADAM12 in cell culture supeatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Disintegrin and metalloproteinase domain-containing protein 12;ADAM 12;3.4.24.-;Meltrin-alpha;ADAM12;MLTN;UNQ346/PRO545;

Molecular Weight

99542 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: R29-S513

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ADAM12

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	285	1490	4755	259	1498	4763
Standard deviation	16.81	75.4%9	256.77	19.68	98.86	338.17
CV (%)	5.4%%	7.1%%	5.4%	7.6%	6.6%	7.1%

Protein Function

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity). .

Background

ADAM12 (Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alteatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	285	300	321	287	298	14.34	4.8%
Sample 2	1490	1376	1529	1374	1442	68.65	4.7%
Sample 3	4755	4542	4639	5146	4770	229.53	4.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	8038
Gene Name	ADAM12
UniProt ID	O43184
Protein Name	Disintegrin and metalloproteinase domain-containing protein 12
Tissue Specificity	Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
Sequence	Contains 1 disintegrin domain.

Applications	ELISA
Research Area	Cancer,Cell Adhesion,Cell Biology,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Integrins,Invasion/Microenvironment,Metalloprotease,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000707	EK0934	96 wells/kit	$499		Add to Cart

Human ADAM12 / MCMP ELISA Kit PicoKine®

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