Human ADA/Adenosine Deaminase ELISA Kit PicoKine®

Mfr.No.

EK1446

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.021	0.078	0.124	0.227	0.398	0.712	1.242	1.802

Description

Human ADA/Adenosine Deaminase ELISA Kit PicoKine® (96 Tests). Quantitate Human ADA in cell culture supeatants, serum and pleural fluid. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Adenosine deaminase;3.5.4.4;Adenosine aminohydrolase;ADA;ADA1;

Molecular Weight

40764 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A2-L363

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ADA

Subcellular Localization

Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen . Cytoplasm . Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.

Sample Type

Cell culture supeatants, Serum, Pleural fluid

Anticoagulant

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	76	257	1026	81	240	1095
Standard deviation	4.63	12.33	41.04	4.01	11.52	53.65
CV (%)	6.1%	4.8%	4%	7.3%	4.8%	4.9%

Protein Function

Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes inly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte- epithelial cell adhesion. .

Background

Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the tuover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	76	69	64	70	69	4.26	6.1%
Sample 2	257	230	240	246	243	9.78	4%
Sample 3	1026	878	895	954	938	57.98	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	100
Gene Name	ADA
UniProt ID	P00813
Protein Name	Adenosine deaminase
Tissue Specificity	Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.

Applications	ELISA
Research Area	Cancer,Cancer Metabolism,Chromatin Modifying Enzymes,Epigenetics and Nuclear Signaling,Metabolism,Metabolism Processes,Pathways and Processes,Response To Hypoxia

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001058	EK1446	96 wells/kit	$499		Add to Cart

Human ADA/Adenosine Deaminase ELISA Kit PicoKine®

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