Human Acylamino-acid-releasing enzyme(APEH) ELISA kit

Mfr.No.

CSB-EL001899HU

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

N-acylaminoacyl-peptide hydrolase, ACPH, APH, D3F15S2, D3S48E, DNF15S2, MGC2178, OPH, acylaminoacyl-peptidase, oxidized protein hydrolase

Recovery

The recovery of human PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-90
EDTA plasma (n=4)	101	97-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	93
	Range %	90-96
1:40	Average %	107
	Range %	103-110
1:80	Average %	86
	Range %	83-90
1:160	Average %	101
	Range %	98-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P13798

Applications	ELISA
Research Area	APEH

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10501044	CSB-EL001899HU	5x96T	$3336	Add to Cart
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