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Home / ELISA Kit

Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) ELISA Kit

SKU : BHE13703102

Fatty acids are incorporated into membranes and signaling molecules and have roles in energy storage and metabolism. These essential functions require activation of the fatty acid by acyl-coenzyme A (CoA) synthetases, such as ACSF2, which form an activating thioester linkage between the fatty acid and CoA.The deduced 615-amino acid protein contains all 5 motifs characteristic of acyl-CoA synthetases. Phylogenetic analysis revealed that ACSF2 belongs to a small group of acyl-CoA synthetases that differ significantly from all other clades of acyl-CoA synthetases and from each other. FLJ20920 contains a PPARG-recognition element in its promoter sequence. Transfection of antisense FLJ20920 into preadipocytes resulted in decreased triglyceride accumulation and reduced expression of GLUT4 (SLC2A4) and HSL (LIPE) mRNA.

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Mfr.No.	KTE60886
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACSF2.
Alternative Names	ACSF2; ACSMW; AVYV493; FLJ20920; PPARG binding; long chain fatty acid acyl Co-A ligase like
Detection Method	Colorimetric
Kit Components	Human Acyl-CoA synthetase family member 2, mitochondrial microplate Human Acyl-CoA synthetase family member 2, mitochondrial standard Human Acyl-CoA synthetase family member 2, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACSF2. No significant cross-reactivity or interference between Human ACSF2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Fatty acids are incorporated into membranes and signaling molecules and have roles in energy storage and metabolism. These essential functions require activation of the fatty acid by acyl-coenzyme A (CoA) synthetases, such as ACSF2, which form an activating thioester linkage between the fatty acid and CoA.The deduced 615-amino acid protein contains all 5 motifs characteristic of acyl-CoA synthetases. Phylogenetic analysis revealed that ACSF2 belongs to a small group of acyl-CoA synthetases that differ significantly from all other clades of acyl-CoA synthetases and from each other. FLJ20920 contains a PPARG-recognition element in its promoter sequence. Transfection of antisense FLJ20920 into preadipocytes resulted in decreased triglyceride accumulation and reduced expression of GLUT4 (SLC2A4) and HSL (LIPE) mRNA.
Notes	This Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACSF2 in samples. An antibody specific for ACSF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACSF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACSF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACSF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	80221

Applications	ELISA
Applications Notes	This Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACSF2 in samples. An antibody specific for ACSF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACSF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACSF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACSF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703102	KTE60886	48 T	$339		Add to Cart
BHE13703102	KTE60886	96 T	$589		Add to Cart
BHE13703102	KTE60886	96 Tx5	$2629		Add to Cart
BHE13703102	KTE60886	96 Tx50	$24569		Add to Cart

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