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Home / ELISA Kit

Human Actin Alpha 1 - Skeletal Muscle - ACTA1 ELISA Kit

SKU : BHE12104509

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

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Mfr.No.

E2972Hu

Sensitivity

0.31ng/ml

Detection Range

0.5-100ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ACTA;ACTa1;Actin, alpha skeletal muscle;Actin, alpha skeletal muscle, intermediate form);Alpha-actin-1;ASMA;CFTD;CFTD1;CFTDM;MPFD;NEM1;NEM2;SHPM;

Molecular Weight

42051

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2972Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	18.03	0.86	4.77
Intra-Assay	2	18	31.39	1.01	3.21
Intra-Assay	3	18	15.69	0.8	5.11

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Protocol

These standard curves of E2972Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.302	2.254	2.223
	2.206
32ng/ml	1.344	1.393	1.362
	1.441
16ng/ml	0.632	0.69	0.659
	0.748
8ng/ml	0.407	0.369	0.338
	0.332
4ng/ml	0.157	0.166	0.135
	0.174
0ng/ml	0.035	0.031	0
	0.026

Gene ID	58
UniProt ID	P68133

Research Area	Tags & Cell Markers

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104509	E2972Hu	48T	$320		Add to Cart
BHE12104509	E2972Hu	96T	$458		Add to Cart

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