Human Acid Sphingomyelinase-like Phosphodiesterase 3B

Mfr.No.

E4893Hu

Alternative Names

Acid sphingomyelinase-like phosphodiesterase 3b;ASM-like phosphodiesterase 3b;SMPDL3B

Reactivity

Human

Detection Range

0.3-90ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

50814

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.14ng/mL

Background

Lipid-modulating phosphodiesterase (PubMed: 26095358). Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling (By similarity). Has in vitro phosphodiesterase activity, but the physiological substrate is unknown (PubMed: 26095358). Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro) (By similarity).

Gene ID	27293
UniProt ID	Q92485
Antigen	SMPDL3B

Research Area

Signal Transduction

Protocol

These standard curves of E4893Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.068	2.195	2.128
	2.321
24ng/mL	1.405	1.411	1.344
	1.416
12ng/mL	0.729	0.779	0.712
	0.828
6ng/mL	0.456	0.48	0.413
	0.505
3ng/mL	0.273	0.285	0.218
	0.297
0ng/mL	0.063	0.067	0
	0.07

Precision

We measured random samples of E4893Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.5	0.55	6.5
Intra-Assay	2	18	17.4	0.97	5.5
Intra-Assay	3	18	25.6	0.94	3.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106166	E4893Hu	48T	$320		Add to Cart
BHE12106166	E4893Hu	96T	$458		Add to Cart

Human Acid Sphingomyelinase-like Phosphodiesterase 3B - SMPDL3B ELISA Kit

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