Human Acetylserotonin O-methyltransferase

Mfr.No.

E6644Hu

Sensitivity

0.016ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Acetylserotonin O-methyltransferase;ASMT;ASMTY;HIOMT;HIOMTY;Hydroxyindole O-methyltransferase

Molecular Weight

38453

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E6644Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.31	0.32	4.35
Intra-Assay	2	18	8.22	0.23	2.75
Intra-Assay	3	18	3.83	0.2	5.35

Background

Isoform 1 catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). Isoform 2 and isoform 3 lack enzyme activity.

Protocol

These standard curves of E6644Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	1.937	1.863	1.82
	1.79
6ng/ml	0.991	0.989	0.946
	0.988
3ng/ml	0.579	0.54	0.497
	0.501
1.5ng/ml	0.154	0.178	0.135
	0.202
0.75ng/ml	0.141	0.125	0.081
	0.109
0ng/ml	0.04	0.044	0
	0.047

Gene ID	438
UniProt ID	P46597

Research Area	Neuroscience; Signal Transduction; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115328	E6644Hu	48T	$320		Add to Cart
BHE12115328	E6644Hu	96T	$458		Add to Cart

Human Acetylserotonin O-methyltransferase - ASMT ELISA Kit

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