Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine®

Mfr.No.

EK1488

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.106	0.121	0.156	0.226	0.404	0.692	1.125	2.009

Description

Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine® (96 Tests). Quantitate Human A1BG in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Alpha-1B-glycoprotein;Alpha-1-B glycoprotein;A1BG;

Molecular Weight

54254 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A22-S495

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human A1BG

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	236	1951	5607	256	1869	5510
Standard deviation	15.81	85.84	409.31	20.48	89.71	495.9
CV (%)	6.7%	4.4%	7.3%	8%	4.8%	9%

Background

Alpha-1-B glycoprotein is a 54.3 kDa protein in humans that is encoded by the A1BG gene. The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. Patients who have pancreatic ductal adenocarcinoma show an overexpression of A1BG in pancreatic juice.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	236	256	258	231	245	11.9	4.8%
Sample 2	1951	1726	1646	1670	1748	120.6	6.8%
Sample 3	5607	5964	6206	6301	6019	267.98	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1
Gene Name	A1BG
UniProt ID	P04217
Protein Name	Alpha-1B-glycoprotein
Tissue Specificity	Plasma.

Applications	ELISA
Research Area	Cell Biology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001258	EK1488	96 wells/kit	$750		Add to Cart

Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine®

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