Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 2

Mfr.No.

E3478Hu

Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 2;ADAMTS 2;ADAM-TS 2;ADAMTS2;ADAMTS-2;ADAM-TS2;PC I-NP;pNPI;Procollagen I N-proteinase;Procollagen I/II amino propeptide-processing enzyme;Procollagen N-endopeptidase

Reactivity

Human

Detection Range

1-400ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

134755

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.53ng/mL

Background

Cleaves the propeptides of type I and II collagen prior to fibril assembly (By similarity). Does not act on type III collagen (By similarity). Cleaves lysyl oxidase LOX at a site downstream of its propeptide cleavage site to produce a short LOX form with reduced collagen-binding activity (PubMed: 31152061).

Gene ID	9509
UniProt ID	O95450
Antigen	ADAMTS2

Research Area

Cardiovascular; Signal Transduction; Cancer; Cell Biology

Protocol

These standard curves of E3478Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/mL	2.35	2.44	2.388
	2.53
120ng/mL	1.532	1.478	1.426
	1.424
60ng/mL	0.776	0.785	0.733
	0.794
30ng/mL	0.381	0.41	0.359
	0.439
15ng/mL	0.238	0.214	0.162
	0.191
0ng/mL	0.061	0.052	0
	0.042

Precision

We measured random samples of E3478Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	42.2	2.19	5.2
Intra-Assay	2	18	91.1	4.51	5
Intra-Assay	3	18	103.1	3.17	3.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104984	E3478Hu	48T	$320		Add to Cart
BHE12104984	E3478Hu	96T	$458		Add to Cart

Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 2 - ADAMTS2 ELISA Kit

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