Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 12

Mfr.No.

E3837Hu

Description

This sandwich kit is for the accurate quantitative detection of human ADAM Metallopeptidase with Thrombospondin Type 1 Motif 12 (also known as ADAMTS12) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 12;ADAMTS 12;ADAM-TS 12;ADAMTS12;ADAMTS-12;ADAM-TS12

Reactivity

Human

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

177676

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.88ng/L

Background

Metalloprotease that may play a role in the degradation of COMP. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties.

Gene ID	81792
UniProt ID	P58397
Antigen	ADAMTS12

Research Area

Signal Transduction

Protocol

These standard curves of E3837Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	1.724	1.721	1.674
	1.718
1600ng/L	0.907	0.933	0.886
	0.959
800ng/L	0.437	0.537	0.49
	0.637
400ng/L	0.201	0.201	0.154
	0.202
200ng/L	0.149	0.123	0.076
	0.098
0ng/L	0.049	0.047	0
	0.045

Precision

We measured random samples of E3837Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2255.7	74.09	3.3
Intra-Assay	2	18	1015.1	59.29	5.8
Intra-Assay	3	18	1280.1	51.99	4.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105282	E3837Hu	48T	$320		Add to Cart
BHE12105282	E3837Hu	96T	$458		Add to Cart

Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 12 - ADAMTS12 ELISA Kit

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