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Home / ELISA Kit

Human 6-keto-prostaglandin F1a (6-K-PGF1a) ELISA Kit

SKU : BHE13703052

6-keto-Prostaglandin F1a is the physiologically active and stable metabolite of prostacyclin. (A prostaglandin found in nearly all mammalian tissue that is a powerful vasodilator and inhibits platelet aggregation; it is biosynthesized enzymatically from prostaglandin endoperoxides in human vascular tissue; the sodium salt has been also used to treat primary pulmonary hypertension (Hypertension, Pulmonary).

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Mfr.No.	KTE60836
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human 6-keto-prostaglandin F1a (6-K-PGF1a) ELISA Kit employs a two-site sandwich ELISA to quantitate 6ketoPGF1a.
Alternative Names	6ketoPGF1a
Detection Method	Colorimetric
Kit Components	Human 6-keto-prostaglandin F1a microplate Human 6-keto-prostaglandin F1a standard Human 6-keto-prostaglandin F1a detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human 6-keto-prostaglandin F1a (6-K-PGF1a) ELISA Kit has high sensitivity and excellent specificity for detection of Human 6ketoPGF1a. No significant cross-reactivity or interference between Human 6ketoPGF1a and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	6-keto-Prostaglandin F1a is the physiologically active and stable metabolite of prostacyclin. (A prostaglandin found in nearly all mammalian tissue that is a powerful vasodilator and inhibits platelet aggregation; it is biosynthesized enzymatically from prostaglandin endoperoxides in human vascular tissue; the sodium salt has been also used to treat primary pulmonary hypertension (Hypertension, Pulmonary).
Notes	This Human 6-keto-prostaglandin F1a (6-K-PGF1a) ELISA Kit employs a two-site sandwich ELISA to quantitate 6ketoPGF1a in samples. An antibody specific for 6ketoPGF1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any6ketoPGF1a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 6ketoPGF1a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 6ketoPGF1a bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human 6-keto-prostaglandin F1a (6-K-PGF1a) ELISA Kit employs a two-site sandwich ELISA to quantitate 6ketoPGF1a in samples. An antibody specific for 6ketoPGF1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any6ketoPGF1a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 6ketoPGF1a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 6ketoPGF1a bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703052	KTE60836	48 T	$359		Add to Cart
BHE13703052	KTE60836	96 T	$619		Add to Cart
BHE13703052	KTE60836	96 Tx5	$2809		Add to Cart
BHE13703052	KTE60836	96 Tx50	$26209		Add to Cart

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