Matrices listed below were spiked with certain level of 4-HNE and the recovery rates were calculated by comparing the measured value to the expected amount of 4-HNE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
93
EDTA plasma(n=5)
90-104
97
heparin plasma(n=5)
85-101
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 4-HNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
4-HNE ELISA Kit allows for the in vitro quantitative determination of 4-HNE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human 4-HNE(4-Hydroxynonenal) ELISA Kit