Human 39S Ribosomal Protein L18 - Mitochondrial

Mfr.No.

E4313hu

Alternative Names

39S ribosomal protein L18, mitochondrial;L18mt;Mitochondrial large ribosomal subunit protein uL18m;MRPL 18;MRPL18;MRP-L18

Reactivity

Human

Detection Range

250-4000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

20577

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

100.8ng/L

Background

Together with thiosulfate sulfurtransferase (TST), acts as a mitochondrial import factor for the cytosolic 5S rRNA. The precursor form shows RNA chaperone activity; is able to fold the 5S rRNA into an import-competent conformation that is recognized by rhodanese (TST). Both the cytoplasmic and mitochondrial forms are able to bind to the helix IV-loop D in the gamma domain of the 5S rRNA.

Gene ID	29074
UniProt ID	Q9H0U6
Antigen	MRPL18

Research Area

Others

Protocol

These standard curves of E4313hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000ng/L	2.193	2.303	2.228
	2.413
2000ng/L	1.42	1.317	1.243
	1.215
1000ng/L	0.705	0.805	0.731
	0.906
500ng/L	0.407	0.472	0.397
	0.537
250ng/L	0.252	0.243	0.168
	0.233
0ng/L	0.075	0.075	0
	0.074

Precision

We measured random samples of E4313hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	693	38.07	5.5
Intra-Assay	2	18	2996.5	104.04	3.5
Intra-Assay	3	18	1440.3	74.17	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105688	E4313hu	48T	$320		Add to Cart
BHE12105688	E4313hu	96T	$458		Add to Cart

Human 39S Ribosomal Protein L18 - Mitochondrial - MRPL18 ELISA Kit

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