Human 3-oxo-5-alpha-steroid 4-dehydrogenase 1

Mfr.No.

E4880Hu

Sensitivity

0.029ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

3-oxo-5-alpha-steroid 4-dehydrogenase 1;S5AR 1;SR type 1;SRD5A1;Steroid 5-alpha-reductase 1

Molecular Weight

29459

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4880Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.37	0.23	3.68
Intra-Assay	2	18	4.5	0.17	3.86
Intra-Assay	3	18	1.91	0.12	6.04

Background

Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

Protocol

These standard curves of E4880Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.233	2.176	2.128
	2.119
6ng/ml	1.386	1.3	1.251
	1.213
3ng/ml	0.61	0.691	0.643
	0.772
1.5ng/ml	0.398	0.404	0.356
	0.411
0.75ng/ml	0.205	0.177	0.129
	0.149
0ng/ml	0.046	0.048	0
	0.051

Gene ID	6715
UniProt ID	P18405

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106153	E4880Hu	48T	$320		Add to Cart
BHE12106153	E4880Hu	96T	$458		Add to Cart

Human 3-oxo-5-alpha-steroid 4-dehydrogenase 1 - SRD5A1 ELISA Kit

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