Human 3-hydroxy-3-methylglutaryl-coenzyme A Synthase 1

Mfr.No.

E0957Hu

Alternative Names

3-hydroxy-3-methylglutaryl-coenzyme A reductase;HMG-CoA reductase;HMGCS1;LDLCQ3

Reactivity

Human

Detection Range

0.2-60ng/mL

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

97476

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.094ng/mL

Background

Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis (PubMed:2991281, PubMed:21357570, PubMed:6995544). HMGCR is the main target of statins, a class of cholesterol-lowering drugs (PubMed:11349148, PubMed:18540668).

Gene ID	3156
UniProt ID	P04035
Antigen	HMGCS1

Research Area

Cardiovascular

Protocol

These standard curves of E0957Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/mL	2.05	2.184	2.138
	2.319
16ng/mL	1.917	1.867	1.821
	1.817
8ng/mL	1.112	1.203	1.157
	1.294
4ng/mL	0.654	0.668	0.621
	0.682
2ng/mL	0.347	0.392	0.345
	0.437
0ng/mL	0.055	0.046	0
	0.038

Precision

We measured random samples of E0957Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.5	0.41	6.33
Intra-Assay	2	18	13.84	0.83	5.97
Intra-Assay	3	18	3.03	0.16	5.32

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12102631	E0957Hu	48T	$320		Add to Cart
BHE12102631	E0957Hu	96T	$458		Add to Cart

Human 3-hydroxy-3-methylglutaryl-coenzyme A Synthase 1 - HMGCS1 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human 3-hydroxy-3-methylglutaryl-coenzyme A Synthase 1 - HMGCS1 ELISA Kit

Rate it now..!!