Human 2-oxoisovalerate Dehydrogenase Subunit Alpha - Mitochondrial

Mfr.No.

E4421Hu

Description

This sandwich kit is for the accurate quantitative detection of human 2-Oxoisovalerate Dehydrogenase Subunit Alpha, Mitochondrial (also known as BCKDHA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial;BCKDE1A;BCKDH E1-alpha;BCKDHA;Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain;MSU;MSUD1;OVD1A

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

50471

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.021ng/ml

Background

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

Gene ID	593
UniProt ID	P12694
Antigen	BCKDHA

Research Area

Others

Protocol

These standard curves of E4421Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.003	2.141	2.107
	2.278
6ng/ml	1.524	1.453	1.42
	1.383
3ng/ml	0.692	0.673	0.639
	0.655
1.5ng/ml	0.307	0.319	0.285
	0.332
0.75ng/ml	0.165	0.187	0.153
	0.209
0ng/ml	0.036	0.034	0
	0.031

Precision

We measured random samples of E4421Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.04	0.13	4.44
Intra-Assay	2	18	3.97	0.11	2.87
Intra-Assay	3	18	7.54	0.26	3.41

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105771	E4421Hu	48T	$320		Add to Cart
BHE12105771	E4421Hu	96T	$458		Add to Cart

Human 2-oxoisovalerate Dehydrogenase Subunit Alpha - Mitochondrial - BCKDHA ELISA Kit

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