Human 1-Phosphatidylinositol-4 - 5-bisphosphate Phosphodiesterase Beta-1

Mfr.No.

E4871Hu

Sensitivity

9.75ng/L

Detection Range

20-6000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1;Phosphoinositide phospholipase C-beta-1;Phospholipase C-beta-1;Phospholipase C-I;PLC-154;PLCB 1;PLCB1;PLC-beta-1;PLC-I;

Molecular Weight

138567

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4871Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2965.7	125.48	4.2
Intra-Assay	2	18	642.1	25.3	3.9
Intra-Assay	3	18	1939.2	87.1	4.5

Background

Catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) and mediates intracellular signaling downstream of G protein-coupled receptors (PubMed: 9188725). Regulates the function of the endothelial barrier.

Protocol

These standard curves of E4871Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	1.872	1.834	1.787
	1.796
1600ng/L	1.051	0.999	0.952
	0.947
800ng/L	0.642	0.526	0.478
	0.41
400ng/L	0.219	0.237	0.19
	0.255
200ng/L	0.073	0.082	0.034
	0.09
0ng/L	0.039	0.047	0
	0.056

Gene ID	23236
UniProt ID	Q9NQ66

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106144	E4871Hu	48T	$320		Add to Cart
BHE12106144	E4871Hu	96T	$458		Add to Cart

Human 1-Phosphatidylinositol-4 - 5-bisphosphate Phosphodiesterase Beta-1 - PLCB1 ELISA Kit

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