HRP-SA (Streptavidin Horseradish Peroxidase) Activity ELISA Kit

Mfr.No.

EU2619

Sensitivity

14uIU/ml

Detection Range

28.125-1800uIU/ml(1800uU=6ng)

Reactivity

Universal

Assay Duration

1 hours

Assay Type

Direct

Alternative Names

HRP-SA ELISA Kit, Streptavidin Horseradish Peroxidase ELISA Kit

Recovery

Add a certain amount of SA-HRP into the sample. Calculate the recovery by comparing the measured value with the expected amount of SA-HRP in the sample.

Sample Type	Recovery Range(%)	Average(%)
serum(n=10)	88-101	96
EDTA plasma(n=10)	92-104	98
Heparin plasma(n=10)	87-99	95

Linearity

Dilute the sample with a certain amount of SA-HRP at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type	1:2	1:4	1:8
serum(n=10)	87-105%	87-97%	85-100%
EDTA plasma(n=10)	93-100%	83-98%	85-98%
Heparin plasma(n=10)	88-98%	82-94%	82-96%

Specificity

Specifically binds with SA-HRP , no obvious cross reaction with other analogues.

Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(uIU/ml)	OD-1	OD-2	Average
0	0.153	0.157	0.155
28.125	0.191	0.197	0.194
56.25	0.281	0.289	0.285
112.5	0.419	0.431	0.425
225	0.597	0.615	0.606
450	0.909	0.935	0.922
900	1.606	1.652	1.629
1800	2.21	2.274	2.242

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (uIU/ml)	57.23	222	892	56.58	226	878
Standard deviation	2.32	10.41	55.57	2.95	12.93	50.22
CV(%)	4.06	4.69	6.23	5.22	5.72	5.72

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5)	37°C for 1 month	2-8°C for 6 months
Average(%)	80	95-100

Notes

For Research Use Only

Usage Notes

Step 1:
Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37°C.

Washing:
Wash the plate twice without immersion.

Step 2:
Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37°C.

Washing:
Wash the plate five times without immersion.

Step 3:
Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)

Step 4:
Add 50ul stop solution. Read at 450nm immediately and calculate.

Antigen	HRP-SA

Applications	ELISA
Applications Notes	HRP-SAActivity ELISA Kit allows for the in vitro quantitative determination of HRP-SA concentrations in serum, plasma, tissue homogenates and other biological fluids.

Storage	2-8°C(Sealed), Don't cryopreserve.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10809208	EU2619	96T	$520		Add to Cart

HRP-SA (Streptavidin Horseradish Peroxidase) Activity ELISA Kit

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