Matrices listed below were spiked with certain level of TF and the recovery rates were calculated by comparing the measured value to the expected amount of TF in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
99
EDTA plasma(n=5)
85-97
90
heparin plasma(n=5)
86-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.