Horse HMGB-2(HighMobility Group Protein B2) ELISA Kit

Mfr.No.

ELK9976

Sensitivity

0.062ng/mL

Detection Range

0.16-10 ng/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HMGB-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	92-101%	82-98%	92-104%	82-96%
EDTA Plasma(n=5)	85-96%	82-92%	86-94%	81-95%
Heparin Plasma(n=5)	95-105%	88-102%	93-101%	81-96%

Standard Curve

Concentration (ng/mL)	OD	Corrected OD
10.00	2.135	2.033
5.00	1.608	1.506
2.50	1.183	1.081
1.25	0.796	0.694
0.63	0.529	0.427
0.32	0.299	0.197
0.16	0.175	0.073
0.00	0.102	0.000

Sample Type

Serum, Plasma and other biological fluids

Limit Of Detection

10 ng/mL

Precision

Matrices listed below were spiked with certain level of recombinant HMGB-2 and the recovery rates were calculated by comparing the measured value to the expected amount of HMGB-2 in samples.

Matrix	Recovery range	Average
serum(n=5)	84-106%	100%
EDTA Plasma(n=5)	82-98%	87%
Heparin Plasma(n=5)	94-107%	100%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse HMGB-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse HMGB-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse HMGB-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse HMGB-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

Research Area	Infection immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15210454	ELK9976	48T	$412		Add to Cart
BHE15210454	ELK9976	96T	$588		Add to Cart

Horse HMGB-2(HighMobility Group Protein B2) ELISA Kit

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