Horse equine TIMP-3 ELISA Kit PicoKine®

Mfr.No.

EK0523-EQ

Sensitivity

<2 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Equine

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.008	0.052	0.079	0.160	0.356	0.826	1.693	2.485

Description

Horse equine TIMP-3 ELISA Kit PicoKine® (96 Tests). Quantitate Horse TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Metalloproteinase inhibitor 3;Protein MIG-5;Tissue inhibitor of metalloproteinases 3;TIMP-3;TIMP3;

Molecular Weight

24145 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: C24-P211

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Subcellular Localization

Secreted, extracellular space, extracellular matrix.

Sample Type

Cell culture supernatants, Serum, Plasma, Saliva

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1250	2687	5353	1430	2832	5657
Standard deviation	72.5	110.2	192.7	95.81	147.3	277.2
CV (%)	5.8	4.1	3.6	6.7	5.2	4.9

Protein Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Background

The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	100033947
Antigen	TIMP-3
Gene Name	TIMP3
UniProt ID	P35625
Protein Name	Metalloproteinase inhibitor 3
Sequence	Belongs to the protease inhibitor I35 (TIMP) family.

Applications	ELISA
Research Area	Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Neuroscience,Protease Inhibitors,Proteolysis/Ubiquitin,Sensory System,Signal Transduction,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001209	EK0523-EQ	96 wells/kit	$499		Add to Cart

Horse equine TIMP-3 ELISA Kit PicoKine®

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