Matrices listed below were spiked with certain level of IL-1 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-99
93
EDTA plasma(n=5)
86-103
94
heparin plasma(n=5)
85-99
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-102%
89-104%
87-105%
EDTA plasma(n=5)
82-97%
84-99%
84-97%
heparin plasma(n=5)
80-92%
85-93%
83-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
A0A1U7Q4M0
Antigen
IL-1
Applications
ELISA
Applications Notes
IL-1 ELISA Kit allows for the in vitro quantitative determination of IL-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.