Goat interleukin 12,IL-12/P40 ELISA KIT

Goat interleukin 12,IL-12/P40 ELISA KIT

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Mfr.No.

CSB-E12103G

Sensitivity

2.34 pg/mL

Detection Range

9.38 pg/mL-600 pg/mL

Reactivity

Goat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), CLMF, CLMF2, IL-12B, NKSF, NKSF2, IL12, subunit p40, interleukin 12, p40, interleukin 12B, interleukin-12 beta chain, natural killer cell stimulatory factor, 40 kD subunit

Recovery

The recovery of pig SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	81-98
EDTA plasma (n=4)	98	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	93
	Range %	87-102
1:4	Average %	97
	Range %	92-102
1:8	Average %	91
	Range %	80-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
320	2.413	2.395	2.404	2.094
160	2.114	2.004	2.059	1.749
80	1.762	1.674	1.718	1.408
40	1.434	1.339	1.387	1.077
20	1.002	0.953	0.978	0.668
10	0.866	0.859	0.863	0.553
5	0.535	0.529	0.532	0.222
0	0.322	0.297	0.310	?

Species

Capra hircus

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P68221

Applications	ELISA
Research Area	IL12B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500761	CSB-E12103G	96T	$595	Add to Cart
BHE10500761	CSB-E12103G	5x96T	$2856	Add to Cart
BHE10500761	CSB-E12103G	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.