Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

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Mfr.No.

CSB-EL006395FI

Sensitivity

75 pg/mL

Detection Range

75 pg/mL-1200 pg/mL

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

cytochrome P450, family 1, subfamily A, polypeptide 1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, OTTHUMP00000183469, aryl hydrocarbon hydroxylase, cytochrome P1-450, dioxin-inducible, cytochrome P450 1A1, cytochrome P450, subfamily I (aromatic compound

Recovery

The recovery of fish C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	95	91-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-100
1:2	Average %	96
	Range %	90-108
1:4	Average %	95
	Range %	91-98
1:8	Average %	95
	Range %	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average	?
500	0.176	0.174	0.175	?
250	0.381	0.393	0.387	?
115	0.557	0.569	0.563	?
37.5	0.894	0.901	0.898	?
12.5	1.481	1.498	1.490	?
0	2.417	2.542	2.480	?

Species

Fish

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q6JZS3

Applications	ELISA
Research Area	CYP1A1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500682	CSB-EL006395FI	96T	$790	Add to Cart
BHE10500682	CSB-EL006395FI	5x96T	$3792	Add to Cart
BHE10500682	CSB-EL006395FI	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.