Chicken Immunoglobulin G (IgG) (Competitive EIA) ELISA Kit

Mfr.No.

CEA544Ga

Sensitivity

95.1ng/mL

Detection Range

246.9-20,000ng/mL

Reactivity

Chicken

Assay Duration

Assay Type

Competitive Inhibition

Description

This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG).
No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Immunoglobulin G (IgG) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G (IgG) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	97-105	101
EDTA plasma(n=5)	89-96	93
heparin plasma(n=5)	98-105	101

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G (IgG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	93-102%	88-104%	86-103%
EDTA plasma(n=5)	99-105%	97-105%	85-96%	81-98%
heparin plasma(n=5)	94-103%	81-98%	82-95%	91-99%

Features

Detects small molecule

Species

Chicken

Sample Type

Cell lysates, Cell culture supernates, Serum, Tissue homogenates, Plasma, Other biological fluids

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Immunoglobulin G (IgG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin G (IgG) and unlabeled Immunoglobulin G (IgG) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G (IgG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Protocol

Datasheet

Applications	ELISA
Research Area	Infection immunity

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Reference	Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88

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SKU	Supplier No.	Size	Your price
BHE10102014	CEA544Ga	96T*1	$430	Add to Cart
BHE10102014	CEA544Ga	96T*5	$1935	Add to Cart
BHE10102014	CEA544Ga	96T*10	$3655	Add to Cart

ELISA Kit for Immunoglobulin G (IgG) (Chicken)

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