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Home / ELISA Kit

EliKine Human Alpha Fetoprotein (AFP) ELISA Kit

SKU : BHE13710216

Alpha-fetoprotein (AFP) is a highly specific and sensitive tumor marker for primary liver cancer. Its Cutoff value is generally 20 ug/L, when more than 500 ug/L or content rising, more certain significance.

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Mfr.No.	KTE6037
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3.5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	EliKine Human Alpha Fetoprotein (AFP) ELISA Kit employs a two-site sandwich ELISA to quantitate Human AFP.
Detection Method	Colorimetric
Kit Components	Human AFP Microplate Human AFP Standard (lyophilized) Sample Diluent (5x) Assay Buffer (5x) HRP-conjugated Human AFP Detect Antibody (100x) HRP Substrate(TMB) Stop Solution Wash Buffer (20x) Plate Covers
Features	EliKine Human Alpha Fetoprotein (AFP) ELISA Kit has high sensitivity and excellent specificity for detection of Human Alpha Fetoprotein (AFP), No significant cross-reactivity or interference between Human Alpha Fetoprotein (AFP) and analogues was observed.
Calibration Range	0.625-40 ng/mL
Sample Type	Cell culture supernatants, Serum, Plasma
Limit Of Detection	0.313 ng/mL
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Alpha-fetoprotein (AFP) is a highly specific and sensitive tumor marker for primary liver cancer. Its Cutoff value is generally 20 ug/L, when more than 500 ug/L or content rising, more certain significance.
Notes	EliKine Human Alpha Fetoprotein (AFP) ELISA Kit employs a double antibody sandwich method to quantitate Human AFP in samples. An antibody specific for Human AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human AFP present is bound by the immobilized antibody. After removing any unbound substances, an HRP-conjugated antibody specific for Human AFP is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Alpha Fetoprotein (AFP) concentration is proportional to the OD450 nm value.
Usage Notes	1. If Sample Diluent (5x) and Assay Buffer (5x) appears to turn yellow or a small amount of precipitation, etc., it is caused by the serum contained in the reagent. Please centrifuge to remove the precipitate, which will not affect normal use. 2. Do not mix or substitute reagents with those from other lots or sources. 3. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. 4. To ensure accurate results, proper adhesion of plate covers during incubation steps is necessary. 5. Stop Solution has certain Corrosive. Please take protective measures when operating.

Applications	ELISA
Applications Notes	EliKine Human Alpha Fetoprotein (AFP) ELISA Kit employs a double antibody sandwich method to quantitate Human AFP in samples. An antibody specific for Human AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human AFP present is bound by the immobilized antibody. After removing any unbound substances, an HRP-conjugated antibody specific for Human AFP is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Alpha Fetoprotein (AFP) concentration is proportional to the OD450 nm value.

Storage Instructions	Stored at 4°C for 12 months.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710216	KTE6037	48 T	$189		Add to Cart
BHE13710216	KTE6037	96 T	$289		Add to Cart

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