Chinese Hamster CXCL14 ELISA Kit PicoKine®

Mfr.No.

EK1285-HA

Sensitivity

<60 pg/ml

Detection Range

125 pg/ml - 4,000 pg/ml

Reactivity

Hamster

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000
O.D.	0.016	0.063	0.118	0.229	0.406	0.790	1.817

Description

Chinese Hamster CXCL14 ELISA Kit PicoKine® (96 Tests). Quantitate Chinese CXCL14 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 60pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-X-C motif chemokine 14;Chemokine BRAK;MIP-2G;Small-inducible cytokine B14;CXCL14;MIP2G, NJAC, SCYB14;PSEC0212, UNQ240/PRO273;

Molecular Weight

13078 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S35-E111

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	987	1638	3285	1387	2694	3525
Standard Deviation	35.5	68.8	187.2	56.87	156	224.2
CV (%)	3.6	4.2	5.7	4.1	5.8	6.4

Protein Function

Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. .

Background

Chemokine (C-X-C motif) ligand 14 (CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK (for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	100754406
Gene Name	CXCL14
UniProt ID	O95715
Protein Name	C-X-C motif chemokine 14
Tissue Specificity	Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte- derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes. .
Sequence	Belongs to the intercrine alpha (chemokine CxC) family.

Applications	ELISA
Research Area	Cancer,Chemokines,Immunology,Innate Immunity,Oncoproteins/Suppressors,Tumor Suppressors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001196	EK1285-HA	96 wells/kit	$450		Add to Cart

Chinese Hamster CXCL14 ELISA Kit PicoKine®

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