Chinese Hamster BMP-7 ELISA Kit PicoKine®

Mfr.No.

EK1443-HA

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Hamster

Sample Volume

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.032	0.143	0.143	0.248	0.423	0.816	1.359	2.231

Description

Chinese Hamster BMP-7 ELISA Kit PicoKine® (96 Tests). Quantitate Chinese Bmp7 in bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Bone morphogenetic protein 7;BMP-7;Osteogenic protein 1;OP-1;Bmp7;Bmp-7, Op1;

Molecular Weight

49199 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: S292-H430

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Subcellular Localization

Secreted .

Sample Type

Bone tissue homogenate, Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	185	665	1408	210	887	1699
Standard deviation	9.25	43.89	66.18	14.91	70.96	132.52
CV (%)	5.0	6.6	4.7	7.1	8.0	7.8

Protein Function

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

Background

Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-?2 superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	100759897
Antigen	BMP-7
Gene Name	Bmp7
UniProt ID	P23359
Protein Name	Bone morphogenetic protein 7

Applications	ELISA
Research Area	Cancer,Cell Biology,Cell Cycle,Cell Differentiation,Cytoskeleton/ECM,Developmental Biology,Extracellular Matrix,Growth Factors/Hormones,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathways,Metabolism,Nervous System Development,Organogenesis,Pathways and Processes,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,Structures,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001197	EK1443-HA	96 wells/kit	$499		Add to Cart

Chinese Hamster BMP-7 ELISA Kit PicoKine®

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