Chicken Osteocalcin - OCN ELISA Kit

Mfr.No.

E0244Ch

Sensitivity

18.25ng/L

Detection Range

30-9000ng/L

Reactivity

Chicken

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Chicken Osteocalcin (also known as OT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BGP;Bone Gla protein;Gamma-carboxyglutamic acid-containing protein;OC;OCN;Osteocalcin;OT

Molecular Weight

10707

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0244Ch within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	929.38	64.03	6.89
Intra-Assay	2	18	1425.58	69.74	4.89
Intra-Assay	3	18	416.73	27.13	6.51

Background

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

Protocol

These standard curves of E0244Ch are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.215	2.139	2.104
	2.063
2400ng/L	1.717	1.696	1.662
	1.676
1200ng/L	1.027	1.076	1.041
	1.124
600ng/L	0.697	0.735	0.7
	0.772
300ng/L	0.474	0.473	0.439
	0.472
0ng/L	0.038	0.034	0
	0.031

Gene ID	396348
UniProt ID	P02822

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101151	E0244Ch	48T	$360		Add to Cart
BHE12101151	E0244Ch	96T	$498		Add to Cart

Chicken Osteocalcin - OCN ELISA Kit

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