Matrices listed below were spiked with certain level of NGF and the recovery rates were calculated by comparing the measured value to the expected amount of NGF in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-99
95
EDTA plasma(n=5)
85-105
96
heparin plasma(n=5)
85-100
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NGF ELISA Kit allows for the in vitro quantitative determination of NGF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Chicken NGF(Nerve Growth Factor) ELISA Kit