Matrices listed below were spiked with certain level of IgY and the recovery rates were calculated by comparing the measured value to the expected amount of IgY in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-105
96
EDTA plasma(n=5)
91-105
97
heparin plasma(n=5)
90-101
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgY and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IgY ELISA Kit allows for the in vitro quantitative determination of IgY concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Chicken IgY(Immunoglobulin Y) ELISA Kit