Matrices listed below were spiked with certain level of ADP/Acrp30 and the recovery rates were calculated by comparing the measured value to the expected amount of ADP/Acrp30 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
94
EDTA plasma(n=5)
86-105
95
heparin plasma(n=5)
85-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADP/Acrp30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ADP/Acrp30 ELISA Kit allows for the in vitro quantitative determination of ADP/Acrp30 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Chicken ADP/Acrp30(Adiponectin) ELISA Kit