Matrices listed below were spiked with certain level of SAA and the recovery rates were calculated by comparing the measured value to the expected amount of SAA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-103
98
EDTA plasma(n=5)
87-104
97
heparin plasma(n=5)
85-105
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
SAA ELISA Kit allows for the in vitro quantitative determination of SAA concentrations in serum, plasma, tissue homogenates and other biological fluids.