Canine Matrix metalloproteinase 13,MMP-13 ELISA kit

The recovery of human ADRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)

Mfr.No.

CSB-E14055c

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Canis familiaris, Dog

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Matrix metalloproteinase 13,MMP-13

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ADRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	104
	Range %	99-110
1:4	Average %	97
	Range %	92-102
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	F1PVS9

Applications	ELISA
Research Area	MMP-13

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease Manuel R. Schmidli.et al,BMC VET RES,May 16

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