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Home / ELISA Kit

Canine Coagulation Factor 8 - F8 ELISA Kit

SKU : BHE12115669

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.

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Bioassay Technology Laboratory

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Sample Request

Mfr.No.

E0425Ca

Sensitivity

0.59ng/ml

Detection Range

1-400ng/ml

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Coagulation factor VIII;F8;Procoagulant component

Molecular Weight

265831

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0425Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	166.7	5.24	3.1
Intra-Assay	2	18	66.6	3.63	5.5
Intra-Assay	3	18	68.6	3.57	5.2

Background

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.

Protocol

These standard curves of E0425Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.104	2.134	2.099
	2.163
120ng/ml	1.189	1.23	1.196
	1.272
60ng/ml	0.691	0.685	0.65
	0.679
30ng/ml	0.39	0.405	0.371
	0.42
15ng/ml	0.186	0.177	0.143
	0.168
0ng/ml	0.051	0.034	0
	0.018

Gene ID	403875
UniProt ID	O18806

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115669	E0425Ca	96T	$475		Add to Cart
BHE12115669	E0425Ca	48T	$350		Add to Cart

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