Matrices listed below were spiked with certain level of C Peptide and the recovery rates were calculated by comparing the measured value to the expected amount of C Peptide in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
98
EDTA plasma(n=5)
87-104
93
heparin plasma(n=5)
86-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C Peptide and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
C PeptideELISA Kit allows for the in vitro quantitative determination of C Peptide concentrations in serum, plasma, tissue homogenates and other biological fluids.